AP09347PU-N

antibody from Acris Antibodies GmbH

Targeting: PCNA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09347PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09347PU-N, RRID:AB_2035832

Product name

anti PCNA

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to an internal region of human PCNA protein

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Xenopus

Host

Rabbit

Vial size

0.1 mg

Concentration

0.62 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot: Affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 µg of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(R)800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: Formalin-Fixed Paraffin-Embedded (FFPE) breast tissue