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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-16576 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal alpha-Galactosidase A/GLA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 ug of Galactosidase alpha antibody [N1C2]. Western blot analysis was performed using Galactosidase alpha antibody [N1C2]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
