NB600-202

antibody from Novus Biologicals

Targeting: TFAP2A AP-2, AP2TF, TFAP2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Radioimmunoassay (Data presented on provider website)

Antibody Data

Product number

NB600-202

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-202, RRID:AB_10003205

Product name

Mouse Monoclonal AP2 alpha Antibody

Antibody type

Monoclonal

Description

Protein A purified. This antibody detects both the alpha and beta forms of AP2.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: AP2 alpha Antibody (A6/2/2) [NB600-202] - An intracellular stain was performed on Hek293 cells with AP2 alpha [A6/2/2] Antibody NB600-202AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.