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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX70163 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70163, RRID:AB_370184
- Product name
- E2F1 antibody [16G7]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references A Synergistic Anti-Cancer Effect of Troglitazone and Lovastatin in a Human Anaplastic Thyroid Cancer Cell Line and in a Mouse Xenograft Model.
Zhong WB, Tsai YC, Chin LH, Tseng JH, Tang LW, Horng S, Fan YC, Hsu SP
International journal of molecular sciences 2018 Jun 22;19(7)
International journal of molecular sciences 2018 Jun 22;19(7)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: THP-1 C: HL-60 10% SDS PAGE GTX70163 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- E2F1 antibody [16G7] detects E2F1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] (GTX70163) diluted at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- E2F1 antibody [16G7] detects E2F1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E2F1 protein stained by E2F1 antibody [16G7] (GTX70163) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using E2F1(GTX70163) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse prostate, using E2F1(GTX70163) antibody at 1:150 dilution. Image courtesy of Ali Kuraishy Postdoc (Laboratory of Dr. Michael Karin, UCSD.)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- E2F1 antibody [16G7] detects E2F1 protein at nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E2F1 antibody [16G7] (GTX70163) diluted at 1:500.
