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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0449 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0449, RRID:AB_982505
- Product name
- E2F1 (phospho S337) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of E2F1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors.
c-Myc-regulated microRNAs modulate E2F1 expression.
Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism.
Joshi B, Ordonez-Ercan D, Dasgupta P, Chellappan S
Oncogene 2005 Mar 24;24(13):2204-17
Oncogene 2005 Mar 24;24(13):2204-17
c-Myc-regulated microRNAs modulate E2F1 expression.
O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT
Nature 2005 Jun 9;435(7043):839-43
Nature 2005 Jun 9;435(7043):839-43
Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism.
Wang C, Hou X, Mohapatra S, Ma Y, Cress WD, Pledger WJ, Chen J
The Journal of biological chemistry 2005 Apr 1;280(13):12339-43
The Journal of biological chemistry 2005 Apr 1;280(13):12339-43
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The E2F1 (phospho S337) polyclonal antibody (Cat # PAB0449) is used in Western blot to detect Phospho-E2F1-S337 in T-47D (left), HepG2 (middle), and A2058 (right) cell lysates
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with E2F1 (phospho S337) polyclonal antibody (Cat # PAB0449) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Product Citations :
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
