685803

antibody from BioLegend

Targeting: FOXA2 HNF3B

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

685803

Provider

BioLegend

Proper citation

BioLegend Cat#685803, RRID:AB_2616800

Product name

Alexa Fluor® 594 anti-FOXA2

Antibody type

Monoclonal

Antigen

Purified recombinant fragment of human FOXA2 expressed in E. Coli.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

P87H4B7

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PC-3 cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml Alexa Fluor® 594 conjugated (red) FOXA2 antibody (clone P87H4B7) in 5% FBS overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye