Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00003170-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003170-M01, RRID:AB_538933
- Product name
- Mouse Monoclonal HNF-3 beta/FoxA2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FOXA2 - forkhead box A2
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Noncoding deletions reveal a gene that is critical for intestinal function.
Generation of human antral and fundic gastric organoids from pluripotent stem cells.
Tumour resistance in induced pluripotent stem cells derived from naked mole-rats.
Oz-Levi D, Olender T, Bar-Joseph I, Zhu Y, Marek-Yagel D, Barozzi I, Osterwalder M, Alkelai A, Ruzzo EK, Han Y, Vos ESM, Reznik-Wolf H, Hartman C, Shamir R, Weiss B, Shapiro R, Pode-Shakked B, Tatarskyy P, Milgrom R, Schvimer M, Barshack I, Imai DM, Coleman-Derr D, Dickel DE, Nord AS, Afzal V, van Bueren KL, Barnes RM, Black BL, Mayhew CN, Kuhar MF, Pitstick A, Tekman M, Stanescu HC, Wells JM, Kleta R, de Laat W, Goldstein DB, Pras E, Visel A, Lancet D, Anikster Y, Pennacchio LA
Nature 2019 Jul;571(7763):107-111
Nature 2019 Jul;571(7763):107-111
Generation of human antral and fundic gastric organoids from pluripotent stem cells.
Broda TR, McCracken KW, Wells JM
Nature protocols 2019 Jan;14(1):28-50
Nature protocols 2019 Jan;14(1):28-50
Tumour resistance in induced pluripotent stem cells derived from naked mole-rats.
Miyawaki S, Kawamura Y, Oiwa Y, Shimizu A, Hachiya T, Bono H, Koya I, Okada Y, Kimura T, Tsuchiya Y, Suzuki S, Onishi N, Kuzumaki N, Matsuzaki Y, Narita M, Ikeda E, Okanoya K, Seino K, Saya H, Okano H, Miura K
Nature communications 2016 May 10;7:11471
Nature communications 2016 May 10;7:11471
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - Analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi ( Cat # H00003170-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody (M01), clone 7E6 (Cat # H00003170-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - Analysis of FOXA2 expression in transfected 293T cell line by FOXA2 monoclonal antibody (M01), clone 7E6.Lane 1: FOXA2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - FOXA2 monoclonal antibody (M01), clone 7E6 Analysis of FOXA2 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HNF-3 beta/FoxA2 Antibody (7E6.) [H00003170-M01] - HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - Detection limit for recombinant GST tagged FOXA2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (7E6) [H00003170-M01] - Analysis of monoclonal antibody to FOXA2 on HepG2 cell. Antibody concentration 10 ug/ml.