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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB2400-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat HNF-3 beta/FoxA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human HNF-3 beta/FoxA2 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y261- Isotype
- IgG
- Antibody clone number
- 2141A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HNF-3 beta/FoxA2 by Western Blot. Western blot shows lysates of human lung tissue and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HNF-3 beta/FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HNF-3 beta/FoxA2 in Human Liver Cancer Tissue. HNF-3 beta/FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HNF-3 beta/FoxA2 in Human iPS Cells differentiated to Mesoderm by Flow Cytometry. Human iPS cells differentiated to Mesoderm (Catalog # SC030B) were stained with Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
