Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- A00758-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-SOX10 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for SOX10 detection. Tested with WB, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Establishment of an astrocyte-like cell line from the brain of tilapia (Oreochromis niloticus) for virus pathogenesis and a vitro model of the blood-brain barrier.
Li Y, Huang Y, Cai J, Jiang D, Jian JC, Lu YS, Wang B
Journal of fish diseases 2022 Oct;45(10):1451-1462
Journal of fish diseases 2022 Oct;45(10):1451-1462
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of U20S cells using anti-SOX10 antibody (A00758-1). Overlay histogram showing U20S cells stained with A00758-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- SOX10 Antibody (A00758-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image