Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- A305-799A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 µg) from Malme-3M, HEK293T, MCF-7, SK-MEL-28, Jurkat, MDA-MB-435, HeLa, and UACC-62 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOX10 antibody (Product # A305-799A-T Lot 2) used for WB at a dilution of 1:1,000. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (Product # A304-523A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse SOX10 by western blot. Samples: Whole cell lysate (15 µg) from TCMK-1, B16-F10, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOX10 antibody (Product # A305-799A-T Lot 2) used for WB at a dilution of 1:1,000. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (Product # A304-523A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 µg) from Malme-3M, Hep-G2, MDA-MB-435, HEK293T, SK-MEL-28, HeLa, UACC-257, Jurkat, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 antibody A305-799A-M (lot A305-799A-M-1) used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SOX10 by immunocytochemistry.Sample: FFPE section of UACC-257 cells.Antibody: Rabbit anti-SOX10 antibody (Product # A305-799A-M; lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SOX10 by immunocytochemistry.Sample: FFPE section of Malme-3M cells.Antibody: Rabbit anti-SOX10 antibody (Product # A305-799A-M lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SOX10 by immunohistochemistry.Sample: FFPE section of breast carcinoma.Antibody: Rabbit anti-SOX10 antibody (Product # A305-799A-M lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SOX10 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-SOX10 antibody (Product # A305-799A-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SOX10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Malme-3M cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOX10 antibody (Product # A305-799A-T Lot 2) used for IP at 20 µL per reaction. SOX10 was also immunoprecipitated by a previous lot of this antibody (Product # A305-799A-T Lot 1) and rabbit anti-SOX10 recombinant monoclonal antibody (Product # A700-080). For blotting immunoprecipitated SOX10 (Product # A700-080) was used at a dilution of 1:1,000. Detection: Chemiluminescence with an exposure time of 30 seconds.