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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA302204 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA302204, RRID:AB_2242527
- Product name
- Rabbit Polyclonal Antibody against AKT2 (N-term)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against AKT2 (N-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- AKT2
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat.#TA302204) in mouse liver tissue lysates (35ug/lane). AKT2(arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat.#TA302204) in 293 cell line lysates transiently transfected with the AKT2 gene (2ug/lane). AKT2(arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of AKT2 Antibody (N-term)(Cat#TA302204) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody (N-term)(Cat.#TA302204), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- AKT2 Antibody (N-term) (Cat. #TA302204) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
