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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ARG51625 - Provider product page
- Provider
- Arigo
- Product name
- anti-Akt2 phospho (Ser474) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) stained with anti-Akt2 (phospho Ser474) antibody ARG51625.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells, treated with serum or calf intestinal phosphatase (CIP), stained with anti-Akt2 (phospho Ser474) antibody ARG51625.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human lung carcinoma tissue stained with anti-Akt2 (phospho Ser474) antibody ARG51625 (left) or the same antibody preincubated with blocking peptide (right).
