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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- BS4022 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS4022, RRID:AB_1663520
- Product name
- Bad (phospho S155) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human Bad around the phosphorylation site of Serine 155.
- Description
- Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-Bad (S155) pAb at 1:500 dilutionLane1:MCF-7 cell lysate treated with colchicineLane2:Raw264.7 cell lysate treated with colchicineLane3:H9C2 cell lysate treated with colchicine
