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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0415 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0415, RRID:AB_982344
- Product name
- BAD (phospho S118) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of BAD.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cooperation of amphiregulin and insulin-like growth factor-1 inhibits Bax- and Bad-mediated apoptosis via a protein kinase C-dependent pathway in non-small cell lung cancer cells.
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis.
A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad.
Hurbin A, Coll JL, Dubrez-Daloz L, Mari B, Auberger P, Brambilla C, Favrot MC
The Journal of biological chemistry 2005 May 20;280(20):19757-67
The Journal of biological chemistry 2005 May 20;280(20):19757-67
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis.
Ying S, Seiffert BM, Häcker G, Fischer SF
Infection and immunity 2005 Mar;73(3):1399-403
Infection and immunity 2005 Mar;73(3):1399-403
A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad.
Antignani A, Youle RJ
Biochemistry 2005 Mar 15;44(10):4074-82
Biochemistry 2005 Mar 15;44(10):4074-82
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The BAD (phospho S118) polyclonal antibody (Cat # PAB0415) is used in Western blot to detect Phospho-BAD-S118 in HL-60 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BAD (phospho S118) polyclonal antibody (Cat # PAB0415) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
