PAB0416

antibody from Abnova Corporation

Targeting: BAD BBC2, BCL2L8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0416

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0416, RRID:AB_982345

Product name

BAD (phospho S134) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of BAD.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

BAD contributes to RAF-mediated proliferation and cooperates with B-RAF-V600E in cancer signaling.

Polzien L, Baljuls A, Albrecht M, Hekman M, Rapp UR
The Journal of biological chemistry 2011 May 20;286(20):17934-44

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Anandamide extends platelets survival through CB(1)-dependent Akt signaling.

Catani MV, Gasperi V, Evangelista D, Finazzi Agrò A, Avigliano L, Maccarrone M
Cellular and molecular life sciences : CMLS 2010 Feb;67(4):601-10

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The BAD (phospho S134) polyclonal antibody (Cat # PAB0416) is used in Western blot to detect Phospho-BAD-S134 in HL-60 cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with BAD (phospho S134) polyclonal antibody (Cat # PAB0416) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)