695502

antibody from BioLegend

Targeting: BAD BBC2, BCL2L8

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

695502

Provider

BioLegend

Proper citation

BioLegend Cat#695502, RRID:AB_2650854

Product name

Purified anti-BAD

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16055A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from HeLa, A431, A20 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 5 µg/mL purified anti-BAD (clone W16055A) antibody. Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.

Conjugate

Unconjugated