M03520

antibody from Boster Biological Technology

Targeting: BAD BBC2, BCL2L8

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M03520

Provider

Boster Biological Technology

Product name

Anti-Bad Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for BAD detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat BAD information: Molecular Weight: 18392 MW; Subcellular Localization: Mitochondrion outer membrane. Cytoplasm . Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm; Tissue Specificity: Expressed in a wide variety of tissues.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

CHC-2

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon cancer, using Bad Antibody(M03520)BAD was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BAD Antibody (M03520)overnight at 4

Additional image