V2005

antibody from NSJ Bioreagents

Targeting: BAX BCL2L4

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

V2005

Provider

NSJ Bioreagents

Product name

Bax Antibody

Antibody type

Monoclonal

Antigen

Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.

Description

Protein G purified antibody

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

2D2

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Primary multiple tumor with affection of the thyroid gland, uterus, urinary bladder, mammary gland and other organs

Romaniuk А, Lyndin M, Smiyanov V, Sikora V, Rieznik A, Kuzenko Y, Budko H, Moskalenko Y, Karpenko L, Sikora V, Gladchenko O
Pathology - Research and Practice 2017 January

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Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis.

Li Y, Ma H, Lu Y, Tan BJ, Xu L, Lawal TO, Mahady GB, Liu D
BioMed research international 2016;2016:2574637

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Characterizing Bcl-2 Family Protein Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction with Native PAGE.

Dewson G
Methods in molecular biology (Clifton, N.J.) 2016;1419:185-96

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Anti- and Pro-apoptotic Bcl2 Proteins Distribution and Metabolic Profile in Human Coronary Aorta Endothelial Cells Before and After HypPDT.

Maslaňáková M, Balogová L, Miškovský P, Tkáčová R, Štroffeková K
Cell biochemistry and biophysics 2016 Sep;74(3):435-47

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Suppression of c-Myc induces apoptosis via an AMPK/mTOR-dependent pathway by 4-O-methyl-ascochlorin in leukemia cells.

Shin JM, Jeong YJ, Cho HJ, Magae J, Bae YS, Chang YC
Apoptosis : an international journal on programmed cell death 2016 May;21(5):657-68

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Maspin-related Orchestration of Aggressiveness of Gastric Cancer.

Gurzu S, Kadar Z, Sugimura H, Orlowska J, Bara T, Bara T Jr, Szederjesi J, Jung I
Applied immunohistochemistry & molecular morphology : AIMM 2016 May-Jun;24(5):326-36

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STAT3 and NF-κB cooperatively control in vitro spontaneous apoptosis and poor chemo-responsiveness in patients with chronic lymphocytic leukemia.

Liu FT, Jia L, Wang P, Wang H, Farren TW, Agrawal SG
Oncotarget 2016 May 31;7(22):32031-45

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Nedaplatin sensitization of cisplatin-resistant human non-small cell lung cancer cells.

Wang H, Zhu X, Huang J, Chen P, Han S, Yan X
Oncology letters 2016 Apr;11(4):2566-2572

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Dynamin-related protein Drp1 is required for Bax translocation to mitochondria in response to irradiation-induced apoptosis.

Wang P, Wang P, Liu B, Zhao J, Pang Q, Agrawal SG, Jia L, Liu FT
Oncotarget 2015 Sep 8;6(26):22598-612

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In non-transformed cells Bak activates upon loss of anti-apoptotic Bcl-XL and Mcl-1 but in the absence of active BH3-only proteins.

Senft D, Weber A, Saathoff F, Berking C, Heppt MV, Kammerbauer C, Rothenfusser S, Kellner S, Kurgyis Z, Besch R, Häcker G
Cell death & disease 2015 Nov 26;6:e1996

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The overexpression and nuclear translocation of Trx-1 during hypoxia confers on HepG2 cells resistance to DDP, and GL-V9 reverses the resistance by suppressing the Trx-1/Ref-1 axis.

Zhao L, Li W, Zhou Y, Zhang Y, Huang S, Xu X, Li Z, Guo Q
Free radical biology & medicine 2015 May;82:29-41

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Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity.

Gurzu S, Kadar Z, Sugimura H, Bara T, Bara T Jr, Halmaciu I, Jung I
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Mar;123(3):223-33

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Oxazoloisoindolinones with in vitro antitumor activity selectively activate a p53-pathway through potential inhibition of the p53-MDM2 interaction.

Soares J, Pereira NA, Monteiro Â, Leão M, Bessa C, Dos Santos DJ, Raimundo L, Queiroz G, Bisio A, Inga A, Pereira C, Santos MM, Saraiva L
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2015 Jan 23;66:138-47

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Enhanced cytotoxicity of prenylated chalcone against tumour cells via disruption of the p53-MDM2 interaction.

Leão M, Soares J, Gomes S, Raimundo L, Ramos H, Bessa C, Queiroz G, Domingos S, Pinto M, Inga A, Cidade H, Saraiva L
Life sciences 2015 Dec 1;142:60-5

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Mcl-1 downregulation leads to the heightened sensitivity exhibited by BCR-ABL positive ALL to induction of energy and ER-stress.

Leclerc GJ, DeSalvo J, Du J, Gao N, Leclerc GM, Lehrman MA, Lampidis TJ, Barredo JC
Leukemia research 2015 Aug 20;

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Role of JNK Activation and Mitochondrial Bax Translocation in Allicin-Induced Apoptosis in Human Ovarian Cancer SKOV3 Cells.

Xu L, Yu J, Zhai D, Zhang D, Shen W, Bai L, Cai Z, Yu C
Evidence-based complementary and alternative medicine : eCAM 2014;2014:378684

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Pro-oxidative action of polyphenols as action mechanism for their pro-apoptotic activity.

Lecci RM, Logrieco A, Leone A
Anti-cancer agents in medicinal chemistry 2014;14(10):1363-75

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Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin.

Charan RA, Johnson BN, Zaganelli S, Nardozzi JD, LaVoie MJ
Cell death & disease 2014 Jul 3;5:e1313

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The novel PI3 kinase inhibitor, BAY 80-6946, impairs melanoma growth in vivo and in vitro.

Schneider P, Schön M, Pletz N, Seitz CS, Liu N, Ziegelbauer K, Zachmann K, Emmert S, Schön MP
Experimental dermatology 2014 Aug;23(8):579-84

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of Hodgkin's lymphoma stained with Bax antibody (Clone 2D2).