sc-17796

antibody from Santa Cruz Biotechnology

Targeting: SMARCA4 BAF190, BRG1, FLJ39786, hSNF2b, SNF2, SNF2-BETA, SNF2L4, SNF2LB, SWI2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-17796

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-17796, RRID:AB_626762

Product name

Anti-SMARCA4

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Diverse roles and interactions of the SWI/SNF chromatin remodeling complex revealed using global approaches.

Euskirchen GM, Auerbach RK, Davidov E, Gianoulis TA, Zhong G, Rozowsky J, Bhardwaj N, Gerstein MB, Snyder M
PLoS genetics 2011 Mar;7(3):e1002008

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Chromatin regulation by Brg1 underlies heart muscle development and disease.

Hang CT, Yang J, Han P, Cheng HL, Shang C, Ashley E, Zhou B, Chang CP
Nature 2010 Jul 1;466(7302):62-7

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Proteomic profiling of nuclei from native renal inner medullary collecting duct cells using LC-MS/MS.

Tchapyjnikov D, Li Y, Pisitkun T, Hoffert JD, Yu MJ, Knepper MA
Physiological genomics 2010 Feb 4;40(3):167-83

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SWI/SNF deficiency results in aberrant chromatin organization, mitotic failure, and diminished proliferative capacity.

Bourgo RJ, Siddiqui H, Fox S, Solomon D, Sansam CG, Yaniv M, Muchardt C, Metzger D, Chambon P, Roberts CW, Knudsen ES
Molecular biology of the cell 2009 Jul;20(14):3192-9

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Coactivation of estrogen receptor beta by gonadotropin-induced cofactor GIOT-4.

Kouzu-Fujita M, Mezaki Y, Sawatsubashi S, Matsumoto T, Yamaoka I, Yano T, Taketani Y, Kitagawa H, Kato S
Molecular and cellular biology 2009 Jan;29(1):83-92

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Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control.

Zhang B, Chambers KJ, Leprince D, Faller DV, Wang S
Oncogene 2009 Feb 5;28(5):651-61

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LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat.

Sachs AN, Pisitkun T, Hoffert JD, Yu MJ, Knepper MA
American journal of physiology. Renal physiology 2008 Dec;295(6):F1799-806

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Analysis of DNA breaks, DNA damage response, and apoptosis produced by high NaCl.

Dmitrieva NI, Burg MB
American journal of physiology. Renal physiology 2008 Dec;295(6):F1678-88

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The reversible epigenetic silencing of BRM: implications for clinical targeted therapy

S Glaros, G M Cirrincione, C Muchardt, C G Kleer, C W Michael, D Reisman
Oncogene 2007 Jun;26(49):7058-7066

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Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region.

Mahajan MC, Narlikar GJ, Boyapaty G, Kingston RE, Weissman SM
Proceedings of the National Academy of Sciences of the United States of America 2005 Oct 18;102(42):15012-7

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Retinoblastoma tumor suppressor: analyses of dynamic behavior in living cells reveal multiple modes of regulation.

Angus SP, Solomon DA, Kuschel L, Hennigan RF, Knudsen ES
Molecular and cellular biology 2003 Nov;23(22):8172-88

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 188

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.