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LearnMA5-31550
antibody from Invitrogen Antibodies
Targeting: SMARCA4
BAF190, BRG1, FLJ39786, hSNF2b, SNF2, SNF2-BETA, SNF2L4, SNF2LB, SWI2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- MA5-31550 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRG1 Monoclonal Antibody (GT2712)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Antibody clone number
- GT2712
- Concentration
- 2.44 mg/mL
Submitted references Expanding the Use of an SS18-SSX Antibody for Molecular Assays in Synovial Sarcoma.
Raquib AR, Hofvander J, Ta M, Nielsen TO
Applied immunohistochemistry & molecular morphology : AIMM 2022 Sep 1;30(8):531-539
Applied immunohistochemistry & molecular morphology : AIMM 2022 Sep 1;30(8):531-539
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRG1 in whole cell extract using BRG1 monoclonal antibody (Product # MA5-31550) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRG1 in various whole cell extracts using BRG1 monoclonal antibody (Product # MA5-31550) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRG1 in various whole cell extracts using BRG1 monoclonal antibody (Product # MA5-31550) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRG1 Monoclonal Antibody (GT2712) detects BRG1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRG1 protein stained by BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRG1 Monoclonal Antibody (GT2712) detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRG1 Monoclonal Antibody (GT2712) detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of BRG1 was performed in frozen-sectioned rat E13.5 brain tissue using BRG1 Monoclonal Antibody (GT2712) (Product # MA5-31550) at a dilution of 1:250 (Green). Red: SOX2, stained by SOX2 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 A Immunofluorescence images of SS18-SSX. Left column: SS18-SSX staining, using an anti-rabbit secondary antibody. Middle column: nuclear counterstaining by 4',6-diamidino-2-phenylindole (DAPI). Last column: merged images. Top row: synovial sarcoma cell line HSSYII. Bottom row: osteosarcoma cell line, U2OS. B Proximity ligation assay between SS18-SSX and BRG1. First column: Red dots indicate close proximity between SS18-SSX and BRG1 proteins within the cells' nuclei. Middle column: single-channel immunofluorescence staining of BRG1 protein. Last column: nuclear counterstain with DAPI. Top row: positive PLA staining in the HSSYII synovial sarcoma cell line. Bottom row: PLA assay performed in the osteosarcoma cell line, U2OS. PLA indicates proximity ligation assay.
