Explore
Validate
Learn21634-1-AP
antibody from Invitrogen Antibodies
Targeting: SMARCA4
BAF190, BRG1, FLJ39786, hSNF2b, SNF2, SNF2-BETA, SNF2L4, SNF2LB, SWI2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 21634-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCA4/BRG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KRPMPGMQQ QMPTLPPPSV SATGPGPGPG PGPGPGPGPA PPNYSRPHGM GGPNMPPPGP SGVPPGMPGQ PPGGPPKPWP EGPMANAAAP TST (207-298 aa encoded by BC150298)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.65 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 293 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH 6.0.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH 6.0.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SMARCA4/BRG1 (IP:21634-1-AP, 5ug; Detection:21634-1-AP 1:1000) with HeLa cells lysate 2500ug.
