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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Flow cytometry [3]
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- Product number
- V2755 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nucleolin Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant human protein was used as the immunogen for the Nucleolin antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Feline, Guinea Pig, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- NCL/902
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of A431 cell lysate using Nucleolin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human uterus stained with Nucleolin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human bladder carcinoma stained with Nucleolin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Nucleolin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human testicular carcinoma stained with Nucleolin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human 293T cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Nucleolin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS: HeLa cells stained with AF488 labeled Nucleolin antibody. Green: AF488-labeled Ab. Blue: DAPI.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry of human Jurkat cells. Grey: isotype control; Green: FITC-labeled; Purple: APC-Labeled; turquoise: AF700-Labeled Nucleolin antibody.
