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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- V2757 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NCL Antibody
- Antibody type
- Monoclonal
- Antigen
- SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 364-5
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the NCL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Nucleolin overexpression in breast cancer cell sub-populations with different stem-like phenotype enables targeted intracellular delivery of synergistic drug combination.
Fonseca NA, Rodrigues AS, Rodrigues-Santos P, Alves V, Gregório AC, Valério-Fernandes Â, Gomes-da-Silva LC, Rosa MS, Moura V, Ramalho-Santos J, Simões S, Moreira JN
Biomaterials 2015 Nov;69:76-88
Biomaterials 2015 Nov;69:76-88
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed paraffin-embedded human skin stained with NCL antibody (364-5).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed paraffin-embedded human tonsil stained with NCL antibody (364-5).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed paraffin-embedded human colon carcinoma stained with AF488 Conjugate of NCL antibody (364-5).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of 293T cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled NCL antibody (364-5).
