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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 10556-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MVKLAKAGK NQGDPKKMAP PPKEVEEDSE DEEMSEDEED DSSGEEVVIP QKKGKKAAAT SAKKVVVSPT KKVAVATPAK KAAVTPGKKA AATPAKKTVT PAKAVTTPGK KGATPGKALV ATPGKKGAAI PAKGAKNGKN AKKEDSDEEE DDDSEEDEED DEDEDEDEDE IEPAAMKAAA AAPASEDEDD EDDEDDEDGK SKGIAYIEFK TEADAEKTFE EKQGTEIDGR SISLYYTGEK GQNQDYRGGK NSTWSGESKT LVLSNLSYSA TEETLQEVFE KATFIKVPQN QNGKSKGYAF IEFASFEDAK EALNSCNKRE IEGRAIRLEL QGPRGSPNAR SQPSKTLFVK GLSEDTTEET LKESFDGSVR ARIVTDRETG SSKGFGFVDF NSEEDAKAAK EAMEDGEIDG NKVTLDWAKP KGEGGFGGRG GGRGGFGGRG GGRGGRGGFG GRGRGGFGGR GGFRGGRGGG GDHKPQGKKT KFE (1-482 aa encoded by BC006494)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.24 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 10556-1-AP (NCL Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10556-1-AP (NCL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (NCL Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (NCL Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 10556-1-AP (NCL antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 10556-1-AP (NCL antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.20 µg NCL antibody (Product # 10556-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NCL (IP:10556-1-AP, 4ug; Detection:10556-1-AP 1:1000) with HeLa cells lysate 3200ug.
