Antibody data
- Product number
- RQ5539 - Provider product page

- Provider
- NSJ Bioreagents
- Product name
- Nucleolin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 100 ug
- Storage
- After reconstitution, the Nucleolin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
NCL protein structure - RQ5539 shown in red.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- Western blot testing of human HepG2 cell lysate with Nucleolin antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- Western blot testing of 1) human K562, 2) human HeLa and 3) monkey COS-7 cell lysate with Nucleolin antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolin antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with Nucleolin antibody (green) and Beta Tubulin antibody (red).
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- IHC staining of FFPE human squamous metaplasia of the renal pelvis with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- IHC staining of FFPE mouse pancreas with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- IHC staining of FFPE rat pancreas with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
-
NSJ Bioreagents (provider)
- Main image

- Experimental details
- Flow cytometry testing of human HL60 cells with Nucleolin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleolin antibody.