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LearnNB600-241
antibody from Novus Biologicals
Targeting: NCL
C23, Nsr1
Western blot
Immunocytochemistry Immunohistochemistry Immunoelectron microscopy Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB600-241 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-241, RRID:AB_10107724
- Product name
- Rabbit Polyclonal Nucleolin Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation.
Identification of Proteomic Markers in Head and Neck Cancer Using MALDI-MS Imaging, LC-MS/MS, and Immunohistochemistry.
Hijacking of multiple phospholipid biosynthetic pathways and induction of membrane biogenesis by a picornaviral 3CD protein.
Deregulation of focal adhesion formation and cytoskeletal tension due to loss of A-type lamins.
Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay.
In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells.
Sustained accumulation of prelamin A and depletion of lamin A/C both cause oxidative stress and mitochondrial dysfunction but induce different cell fates.
Topical application of a G-Quartet aptamer targeting nucleolin attenuates choroidal neovascularization in a model of age-related macular degeneration.
Notch is a direct negative regulator of the DNA-damage response.
Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor β transcription in breast cancer cells.
Nucleolin level in plant root meristematic cells under chilling stress and recovery.
Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.
Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis.
Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein.
Differential effect of membrane cholesterol removal on mu- and delta-opioid receptors: a parallel comparison of acute and chronic signaling to adenylyl cyclase.
Viral regulation of aquaporin 4, connexin 43, microcephalin and nucleolin.
Identification of novel proteins associated with both alpha-synuclein and DJ-1.
Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern.
Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin.
Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1.
Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1.
Okur MN, Lee JH, Osmani W, Kimura R, Demarest TG, Croteau DL, Bohr VA
Nucleic acids research 2020 Mar 18;48(5):2473-2485
Nucleic acids research 2020 Mar 18;48(5):2473-2485
Identification of Proteomic Markers in Head and Neck Cancer Using MALDI-MS Imaging, LC-MS/MS, and Immunohistochemistry.
Hoffmann F, Umbreit C, Krüger T, Pelzel D, Ernst G, Kniemeyer O, Guntinas-Lichius O, Berndt A, von Eggeling F
Proteomics. Clinical applications 2019 Jan;13(1):e1700173
Proteomics. Clinical applications 2019 Jan;13(1):e1700173
Hijacking of multiple phospholipid biosynthetic pathways and induction of membrane biogenesis by a picornaviral 3CD protein.
Banerjee S, Aponte-Diaz D, Yeager C, Sharma SD, Ning G, Oh HS, Han Q, Umeda M, Hara Y, Wang RYL, Cameron CE
PLoS pathogens 2018 May;14(5):e1007086
PLoS pathogens 2018 May;14(5):e1007086
Deregulation of focal adhesion formation and cytoskeletal tension due to loss of A-type lamins.
Corne TDJ, Sieprath T, Vandenbussche J, Mohammed D, Te Lindert M, Gevaert K, Gabriele S, Wolf K, De Vos WH
Cell adhesion & migration 2017 Sep 3;11(5-6):447-463
Cell adhesion & migration 2017 Sep 3;11(5-6):447-463
Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay.
Park OH, Park J, Yu M, An HT, Ko J, Kim YK
Genes & development 2016 Sep 15;30(18):2093-2105
Genes & development 2016 Sep 15;30(18):2093-2105
In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells.
Robijns J, Molenberghs F, Sieprath T, Corne TD, Verschuuren M, De Vos WH
Scientific reports 2016 Jul 27;6:30325
Scientific reports 2016 Jul 27;6:30325
Sustained accumulation of prelamin A and depletion of lamin A/C both cause oxidative stress and mitochondrial dysfunction but induce different cell fates.
Sieprath T, Corne TD, Nooteboom M, Grootaert C, Rajkovic A, Buysschaert B, Robijns J, Broers JL, Ramaekers FC, Koopman WJ, Willems PH, De Vos WH
Nucleus (Austin, Tex.) 2015;6(3):236-46
Nucleus (Austin, Tex.) 2015;6(3):236-46
Topical application of a G-Quartet aptamer targeting nucleolin attenuates choroidal neovascularization in a model of age-related macular degeneration.
Leaderer D, Cashman SM, Kumar-Singh R
Experimental eye research 2015 Nov;140:171-178
Experimental eye research 2015 Nov;140:171-178
Notch is a direct negative regulator of the DNA-damage response.
Vermezovic J, Adamowicz M, Santarpia L, Rustighi A, Forcato M, Lucano C, Massimiliano L, Costanzo V, Bicciato S, Del Sal G, d'Adda di Fagagna F
Nature structural & molecular biology 2015 May;22(5):417-24
Nature structural & molecular biology 2015 May;22(5):417-24
Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor β transcription in breast cancer cells.
Litchfield LM, Riggs KA, Hockenberry AM, Oliver LD, Barnhart KG, Cai J, Pierce WM Jr, Ivanova MM, Bates PJ, Appana SN, Datta S, Kulesza P, McBryan J, Young LS, Klinge CM
PloS one 2012;7(5):e38278
PloS one 2012;7(5):e38278
Nucleolin level in plant root meristematic cells under chilling stress and recovery.
Stępiński D
Micron (Oxford, England : 1993) 2012 Aug;43(8):870-5
Micron (Oxford, England : 1993) 2012 Aug;43(8):870-5
Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.
Kume K, Iizumi Y, Shimada M, Ito Y, Kishi T, Yamaguchi Y, Handa H
Genes to cells : devoted to molecular & cellular mechanisms 2010 Apr 1;15(4):339-49
Genes to cells : devoted to molecular & cellular mechanisms 2010 Apr 1;15(4):339-49
Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis.
Drygin D, Siddiqui-Jain A, O'Brien S, Schwaebe M, Lin A, Bliesath J, Ho CB, Proffitt C, Trent K, Whitten JP, Lim JK, Von Hoff D, Anderes K, Rice WG
Cancer research 2009 Oct 1;69(19):7653-61
Cancer research 2009 Oct 1;69(19):7653-61
Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein.
González V, Guo K, Hurley L, Sun D
The Journal of biological chemistry 2009 Aug 28;284(35):23622-35
The Journal of biological chemistry 2009 Aug 28;284(35):23622-35
Differential effect of membrane cholesterol removal on mu- and delta-opioid receptors: a parallel comparison of acute and chronic signaling to adenylyl cyclase.
Levitt ES, Clark MJ, Jenkins PM, Martens JR, Traynor JR
The Journal of biological chemistry 2009 Aug 14;284(33):22108-22
The Journal of biological chemistry 2009 Aug 14;284(33):22108-22
Viral regulation of aquaporin 4, connexin 43, microcephalin and nucleolin.
Fatemi SH, Folsom TD, Reutiman TJ, Sidwell RW
Schizophrenia research 2008 Jan;98(1-3):163-77
Schizophrenia research 2008 Jan;98(1-3):163-77
Identification of novel proteins associated with both alpha-synuclein and DJ-1.
Jin J, Li GJ, Davis J, Zhu D, Wang Y, Pan C, Zhang J
Molecular & cellular proteomics : MCP 2007 May;6(5):845-59
Molecular & cellular proteomics : MCP 2007 May;6(5):845-59
Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern.
Novoselov SV, Kryukov GV, Xu XM, Carlson BA, Hatfield DL, Gladyshev VN
The Journal of biological chemistry 2007 Apr 20;282(16):11960-8
The Journal of biological chemistry 2007 Apr 20;282(16):11960-8
Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin.
Huddleson JP, Ahmad N, Lingrel JB
The Journal of biological chemistry 2006 Jun 2;281(22):15121-8
The Journal of biological chemistry 2006 Jun 2;281(22):15121-8
Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1.
Hanakahi LA, Dempsey LA, Li MJ, Maizels N
Proceedings of the National Academy of Sciences of the United States of America 1997 Apr 15;94(8):3605-10
Proceedings of the National Academy of Sciences of the United States of America 1997 Apr 15;94(8):3605-10
Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1.
Hanakahi LA, Dempsey LA, Li MJ, Maizels N
Proceedings of the National Academy of Sciences of the United States of America 1997 Apr 15;94(8):3605-10
Proceedings of the National Academy of Sciences of the United States of America 1997 Apr 15;94(8):3605-10
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Nucleolin Antibody [NB600-241] - Image shows a specific band for Nucleolin in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Nucleolin Antibody [NB600-241] - Detection of Nucleolin in human tonsil germinal center.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). Image from verified customer review.
