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- References [4]
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- Western blot [2]
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- Product number
- ABIN953773 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Nucleolin (NCL) (AA 435-463), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of Human Nucleolin. Genename: NCL
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 435-463,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references BRCA1 protein and nucleolin colocalize in breast carcinoma tissue and cancer cell lines.
Conserved residues in the UL24 protein of herpes simplex virus 1 are important for dispersal of the nucleolar protein nucleolin.
Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication.
Mechanism of regulation of bcl-2 mRNA by nucleolin and A+U-rich element-binding factor 1 (AUF1).
Tulchin N, Chambon M, Juan G, Dikman S, Strauchen J, Ornstein L, Billack B, Woods NT, Monteiro AN
The American journal of pathology 2010 Mar;176(3):1203-14
The American journal of pathology 2010 Mar;176(3):1203-14
Conserved residues in the UL24 protein of herpes simplex virus 1 are important for dispersal of the nucleolar protein nucleolin.
Bertrand L, Leiva-Torres GA, Hyjazie H, Pearson A
Journal of virology 2010 Jan;84(1):109-18
Journal of virology 2010 Jan;84(1):109-18
Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication.
Strang BL, Boulant S, Coen DM
Journal of virology 2010 Feb;84(4):1771-84
Journal of virology 2010 Feb;84(4):1771-84
Mechanism of regulation of bcl-2 mRNA by nucleolin and A+U-rich element-binding factor 1 (AUF1).
Ishimaru D, Zuraw L, Ramalingam S, Sengupta TK, Bandyopadhyay S, Reuben A, Fernandes DJ, Spicer EK
The Journal of biological chemistry 2010 Aug 27;285(35):27182-91
The Journal of biological chemistry 2010 Aug 27;285(35):27182-91
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of NCL (arrow) using Nucleolin Antibody (Center) Cat.-No AP52825PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Nucleolin Antibody (Center) Cat.-No AP52825PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the NCL antibody detected the NCL protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Nucleolin Antibody (Center) Cat.-No AP52825PU-N with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human breast tissue stained with Nucleolin Antibody (Center) Cat.-No AP52825PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
