HPA001530

antibody from Atlas Antibodies

Targeting: ARMCX3 ALEX3, GASP6

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA001530

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001530, RRID:AB_2666349

Product name

Anti-ARMCX3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

MAEGGSGDVDDAGDCSGARYNDWSDDDDDSNESKS
IVWYPPWARIGTEAGTRARARARARATRARRAVQK
RASPNSDDTVLSPQELQKVLCLVEMSEKPYILEAA
LIALGNNAAYAFNRDIIRDLGGLPIVAKILNTR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and PC-3 using Anti-ARMCX3 antibody. Corresponding ARMCX3 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and ARMCX3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406053).

Sample type

HUMAN

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.

Sample type

HUMAN