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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3405 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3405, RRID:AB_1574437
- Product name
- DOK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DOK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors.
Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling.
Molecular cloning of a truncated p62Dok1 isoform, p22Dok(del).
Yamakawa N, Tsuchida K, Sugino H
The EMBO journal 2002 Apr 2;21(7):1684-94
The EMBO journal 2002 Apr 2;21(7):1684-94
Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling.
Liang X, Wisniewski D, Strife A, Shivakrupa, Clarkson B, Resh MD
The Journal of biological chemistry 2002 Apr 19;277(16):13732-8
The Journal of biological chemistry 2002 Apr 19;277(16):13732-8
Molecular cloning of a truncated p62Dok1 isoform, p22Dok(del).
Hubert P, Ferreira V, Debre P, Bismuth G
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics 2000 Jun;27(3):145-8
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics 2000 Jun;27(3):145-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of DOK1 polyclonal antibody (Cat # PAB3405) in HL-60 cell lysate. DOK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DOK1 polyclonal antibody (Cat # PAB3405) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
