CABT-B9277

antibody from Creative Diagnostics

Targeting: DOK1 p62dok

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9277

Provider

Creative Diagnostics

Product name

Mouse anti-Human p62/Dok monoclonal antibody, clone 56/q73[epl]

Antibody type

Monoclonal

Description

p62 [Dok] (Downstream of tyrosine kinases) was identified as a target of protein tyrosine kinases. Following phosphorylation, p62 [Dok] binds to Ras GTPase activating protein (Ras-GAP), indicating a role for p62 [Dok] in intracellular signaling pathways. p62 [Dok] contains several motifs that signify its important interactions with signaling proteins. These domains include the pleckstrin homology (PH) domain in the amino terminus, numerous tyrosines in the C-terminus, and ten PXXP motifs. p62 [Dok] localizes to the cell membrane by binding to inositol phosphates via its PH domain. When phosphorylated, the tyrosines serve as binding sites for SH2 containing proteins and the polyproline regions serve as binding sites for SH3 containing proteins. p62 [Dok] is constitutively phosphorylated in CML patients, suggesting that it is a target of the translocation induced increase in tyrosine kinase activity of c-Abl. In addition, p62 [Dok] is phosphorylated following c-Kit ligand binding to the c-Kit receptor.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

56/q73[epl]

Vial size

50 _g

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.