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Learnsc-1647
antibody from Santa Cruz Biotechnology
Targeting: MAPK1
ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-1647 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-1647, RRID:AB_627547
- Product name
- Anti-MAPK1
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
Submitted references Genomic collaboration of estrogen receptor alpha and extracellular signal-regulated kinase 2 in regulating gene and proliferation programs.
BRAF inactivation drives aneuploidy by deregulating CRAF.
Reduction of IKKalpha expression promotes chronic ultraviolet B exposure-induced skin inflammation and carcinogenesis.
Novel nuclear nesprin-2 variants tether active extracellular signal-regulated MAPK1 and MAPK2 at promyelocytic leukemia protein nuclear bodies and act to regulate smooth muscle cell proliferation.
Oscillatory flow-induced proliferation of osteoblast-like cells is mediated by alphavbeta3 and beta1 integrins through synergistic interactions of focal adhesion kinase and Shc with phosphatidylinositol 3-kinase and the Akt/mTOR/p70S6K pathway.
Isoproterenol and cAMP block ERK phosphorylation and enhance [Ca2+]i increases and oxygen consumption by muscarinic receptor stimulation in rat parotid and submandibular acinar cells.
PI3-kinase-dependent activation of apoptotic machinery occurs on commitment of epidermal keratinocytes to terminal differentiation.
The caspase-cleaved form of LYN mediates a psoriasis-like inflammatory syndrome in mice.
DeltaNp63 is essential for epidermal commitment of embryonic stem cells.
Regulation of ERK1/2 by ouabain and Na-K-ATPase-dependent energy utilization and AMPK activation in parotid acinar cells.
Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope.
Mechanisms of induction of endothelial cell E-selectin expression by smooth muscle cells and its inhibition by shear stress.
Deletion of the COOH-terminal domain of CXC chemokine receptor 4 leads to the down-regulation of cell-to-cell contact, enhanced motility and proliferation in breast carcinoma cells.
Catalytically inactive human cathepsin D triggers fibroblast invasive growth.
Autocrine activation of an osteopontin-CD44-Rac pathway enhances invasion and transformation by H-RasV12
Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis.
Borna disease virus nucleoprotein interacts with the CDC2-cyclin B1 complex.
Angiotensin II stimulates hyperplasia but not hypertrophy in immature ovine cardiomyocytes.
p19ARF-independent induction of p53 and cell cycle arrest by Raf in murine keratinocytes.
A role for mitogen-activated protein kinase activation by integrins in the pathogenesis of psoriasis.
Madak-Erdogan Z, Lupien M, Stossi F, Brown M, Katzenellenbogen BS
Molecular and cellular biology 2011 Jan;31(1):226-36
Molecular and cellular biology 2011 Jan;31(1):226-36
BRAF inactivation drives aneuploidy by deregulating CRAF.
Kamata T, Hussain J, Giblett S, Hayward R, Marais R, Pritchard C
Cancer research 2010 Nov 1;70(21):8475-86
Cancer research 2010 Nov 1;70(21):8475-86
Reduction of IKKalpha expression promotes chronic ultraviolet B exposure-induced skin inflammation and carcinogenesis.
Xia X, Park E, Liu B, Willette-Brown J, Gong W, Wang J, Mitchell D, Fischer SM, Hu Y
The American journal of pathology 2010 May;176(5):2500-8
The American journal of pathology 2010 May;176(5):2500-8
Novel nuclear nesprin-2 variants tether active extracellular signal-regulated MAPK1 and MAPK2 at promyelocytic leukemia protein nuclear bodies and act to regulate smooth muscle cell proliferation.
Warren DT, Tajsic T, Mellad JA, Searles R, Zhang Q, Shanahan CM
The Journal of biological chemistry 2010 Jan 8;285(2):1311-20
The Journal of biological chemistry 2010 Jan 8;285(2):1311-20
Oscillatory flow-induced proliferation of osteoblast-like cells is mediated by alphavbeta3 and beta1 integrins through synergistic interactions of focal adhesion kinase and Shc with phosphatidylinositol 3-kinase and the Akt/mTOR/p70S6K pathway.
Lee DY, Li YS, Chang SF, Zhou J, Ho HM, Chiu JJ, Chien S
The Journal of biological chemistry 2010 Jan 1;285(1):30-42
The Journal of biological chemistry 2010 Jan 1;285(1):30-42
Isoproterenol and cAMP block ERK phosphorylation and enhance [Ca2+]i increases and oxygen consumption by muscarinic receptor stimulation in rat parotid and submandibular acinar cells.
Soltoff SP, Hedden L
The Journal of biological chemistry 2010 Apr 30;285(18):13337-48
The Journal of biological chemistry 2010 Apr 30;285(18):13337-48
PI3-kinase-dependent activation of apoptotic machinery occurs on commitment of epidermal keratinocytes to terminal differentiation.
Janes SM, Ofstad TA, Campbell DH, Eddaoudi A, Warnes G, Davies D, Watt FM
Cell research 2009 Mar;19(3):328-39
Cell research 2009 Mar;19(3):328-39
The caspase-cleaved form of LYN mediates a psoriasis-like inflammatory syndrome in mice.
Marchetti S, Gamas P, Belhacène N, Grosso S, Pradelli LA, Colosetti P, Johansen C, Iversen L, Deckert M, Luciano F, Hofman P, Ortonne N, Khemis A, Mari B, Ortonne JP, Ricci JE, Auberger P
The EMBO journal 2009 Aug 19;28(16):2449-60
The EMBO journal 2009 Aug 19;28(16):2449-60
DeltaNp63 is essential for epidermal commitment of embryonic stem cells.
Medawar A, Virolle T, Rostagno P, de la Forest-Divonne S, Gambaro K, Rouleau M, Aberdam D
PloS one 2008;3(10):e3441
PloS one 2008;3(10):e3441
Regulation of ERK1/2 by ouabain and Na-K-ATPase-dependent energy utilization and AMPK activation in parotid acinar cells.
Soltoff SP, Hedden L
American journal of physiology. Cell physiology 2008 Sep;295(3):C590-9
American journal of physiology. Cell physiology 2008 Sep;295(3):C590-9
Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope.
González JM, Navarro-Puche A, Casar B, Crespo P, Andrés V
The Journal of cell biology 2008 Nov 17;183(4):653-66
The Journal of cell biology 2008 Nov 17;183(4):653-66
Mechanisms of induction of endothelial cell E-selectin expression by smooth muscle cells and its inhibition by shear stress.
Chiu JJ, Chen LJ, Lee CI, Lee PL, Lee DY, Tsai MC, Lin CW, Usami S, Chien S
Blood 2007 Jul 15;110(2):519-28
Blood 2007 Jul 15;110(2):519-28
Deletion of the COOH-terminal domain of CXC chemokine receptor 4 leads to the down-regulation of cell-to-cell contact, enhanced motility and proliferation in breast carcinoma cells.
Ueda Y, Neel NF, Schutyser E, Raman D, Richmond A
Cancer research 2006 Jun 1;66(11):5665-75
Cancer research 2006 Jun 1;66(11):5665-75
Catalytically inactive human cathepsin D triggers fibroblast invasive growth.
Laurent-Matha V, Maruani-Herrmann S, Prébois C, Beaujouin M, Glondu M, Noël A, Alvarez-Gonzalez ML, Blacher S, Coopman P, Baghdiguian S, Gilles C, Loncarek J, Freiss G, Vignon F, Liaudet-Coopman E
The Journal of cell biology 2005 Jan 31;168(3):489-99
The Journal of cell biology 2005 Jan 31;168(3):489-99
Autocrine activation of an osteopontin-CD44-Rac pathway enhances invasion and transformation by H-RasV12
Hidemi Teramoto, Maria Domenica Castellone, Renae L Malek, Noah Letwin, Bryan Frank, J Silvio Gutkind, Norman H Lee
Oncogene 2004 Nov;24(3):489-501
Oncogene 2004 Nov;24(3):489-501
Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis.
Janes SM, Watt FM
The Journal of cell biology 2004 Aug 2;166(3):419-31
The Journal of cell biology 2004 Aug 2;166(3):419-31
Borna disease virus nucleoprotein interacts with the CDC2-cyclin B1 complex.
Planz O, Pleschka S, Oesterle K, Berberich-Siebelt F, Ehrhardt C, Stitz L, Ludwig S
Journal of virology 2003 Oct;77(20):11186-92
Journal of virology 2003 Oct;77(20):11186-92
Angiotensin II stimulates hyperplasia but not hypertrophy in immature ovine cardiomyocytes.
Sundgren NC, Giraud GD, Stork PJ, Maylie JG, Thornburg KL
The Journal of physiology 2003 May 1;548(Pt 3):881-91
The Journal of physiology 2003 May 1;548(Pt 3):881-91
p19ARF-independent induction of p53 and cell cycle arrest by Raf in murine keratinocytes.
Roper E, Weinberg W, Watt FM, Land H
EMBO reports 2001 Feb;2(2):145-50
EMBO reports 2001 Feb;2(2):145-50
A role for mitogen-activated protein kinase activation by integrins in the pathogenesis of psoriasis.
Haase I, Hobbs RM, Romero MR, Broad S, Watt FM
The Journal of clinical investigation 2001 Aug;108(4):527-36
The Journal of clinical investigation 2001 Aug;108(4):527-36
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- A-431
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 41
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
