GTX17942

antibody from GeneTex

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX17942

Provider

GeneTex

Proper citation

GeneTex Cat#GTX17942, RRID:AB_422985

Product name

ERK1/2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

References

Submitted references

A new water soluble MAPK activator exerts antitumor activity in melanoma cells resistant to the BRAF inhibitor vemurafenib.

Graziani G, Artuso S, De Luca A, Muzi A, Rotili D, Scimeca M, Atzori MG, Ceci C, Mai A, Leonetti C, Levati L, Bonanno E, Tentori L, Caccuri AM
Biochemical pharmacology 2015 May 1;95(1):16-27

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of PC12 cells unstimulated (-) or stimulated with 20 ng/ml NGF for 20 minutes (+) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4 degree C, then incubated with 0.5 μg/ml ERK1&2 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that the ERK1&2 antibody allows the total amount of ERK1&2 to be measured (non-phoshphorylated as well as phosphorylated). The resulting signals serve as useful controls in assessing the degree of up-regulation as detected using an antibody to the dually phosphorylated (active) form of ERK1&2