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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- ARG10568 - Provider product page
- Provider
- Arigo
- Product name
- anti-Erk1/2 antibody [B20-U]
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide around the C-terminus of Human Erk 2
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- B20-U
- Vial size
- 50 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 100 µg of Rat brain crude lysate stained with ARG10568 anti-Erk1/2 antibody [B20-U].
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HEK293 cells stained with ARG10568 anti-Erk1/2 antibody [B20-U] at 1:200 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: Erk 1,2 immunoprecipitated from HEK 293 cells, visualized with ARG10568 anti-Erk1/2 antibody [B20-U] in WB analysis at 1:5000 dilution. 1) Marker, 2) 100 µg of HEK 293 stained with ARG10568 anti-Erk1/2 antibody [B20-U] (positive control), 3) Immunoprecipitation without primary antibody (negative control), and 4) Immunoprecipitated Erk 1,2 (~40 and ~45 kDa proteins) from HEK 293 cells (500 µg of crude protein extract) stained with ARG10568 anti-Erk1/2 antibody [B20-U].
