MAB1230

antibody from R&D Systems

Targeting: MAPK1 ERK, ERK2, MAPK2, p41mapk, PRKM1, PRKM2

Immunohistochemistry

Antibody data

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Validation data

Product number: MAB1230 - Provider product page
Provider: R&D Systems
Product name: Human/Mouse/Rat ERK2 Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from hybridoma culture supernatant. Detects human, rat and mouse ERK2. This antibody does not recognize endogenous ERK1.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: Q1HBJ4
Isotype: IgG
Antibody clone number: 191801
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

MAPK1 protein structure - MAB1230 shown in red.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human/Mouse/Rat ERK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERK2 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human ERK2 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for ERK2 at approximately 43 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Western Blot Shows Human ERK2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.