10723-1-AP

antibody from Proteintech Group

Targeting: XRCC6 D22S671, D22S731, G22P1, KU70, ML8

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10723-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10723-1-AP, RRID:AB_2218756

Product name

KU70 antibody

Antibody type

Polyclonal

Description

KD/KO validated KU70 antibody (Cat. #10723-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability.

Cao C, Han P, Liu L, Tang Y, Tian S, Zhang K, Shi L, Liu Z, Zhuo D, Ge W, Gong W
The Journal of biological chemistry 2021 Sep;297(3):101036

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Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca²⁺]_m-PDP1-PDH-histone acetylation retrograde signaling.

Shi Y, Wang Y, Jiang H, Sun X, Xu H, Wei X, Wei Y, Xiao G, Song Z, Zhou F
Cell death & disease 2021 Sep 6;12(9):837

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OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.

Cui Y, Xie R, Zhang X, Liu Y, Hu Y, Li Y, Liu X, Yu X, Wu C
Cell death & disease 2021 Jun 16;12(7):622

---

LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma.

Guo Z, Wang YH, Xu H, Yuan CS, Zhou HH, Huang WH, Wang H, Zhang W
Cell death & disease 2021 Jan 4;12(1):69

---

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Li G, Zhang X, Wang H, Liu D, Li Z, Wu Z, Yang H
The international journal of biochemistry & cell biology 2020 Aug;125:105790

---

Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.

Aiken CE, Tarry-Adkins JL, Spiroski AM, Nuzzo AM, Ashmore TJ, Rolfo A, Sutherland MJ, Camm EJ, Giussani DA, Ozanne SE
The Journal of physiology 2019 May;597(9):2391-2401

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Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Wang Y, Hu L, Zhang X, Zhao H, Xu H, Wei Y, Jiang H, Xie C, Zhou Y, Zhou F
Journal of Cancer 2017;8(8):1400-1409

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USP22 Induces Cisplatin Resistance in Lung Adenocarcinoma by Regulating γH2AX-Mediated DNA Damage Repair and Ku70/Bax-Mediated Apoptosis.

Wang A, Ning Z, Lu C, Gao W, Liang J, Yan Q, Tan G, Liu J
Frontiers in pharmacology 2017;8:274

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UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Hu L, Li X, Liu Q, Xu J, Ge H, Wang Z, Wang H, Wang Z, Shi C, Xu X, Huang J, Lin Z, Pieper RO, Weng C
Oncogene 2017 Feb 23;36(8):1145-1156

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High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the β-Catenin/Wnt Signaling Pathway and Is Associated with Poor Prognosis.

Zhu B, Cheng D, Li S, Zhou S, Yang Q
International journal of molecular sciences 2016 Jul 22;17(7)

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Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Dong H, Shi Q, Song X, Fu J, Hu L, Xu D, Su C, Xia X, Song E, Song Y
Toxicology and applied pharmacology 2015 Jul 1;286(1):10-6

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RNAi silencing targeting RNF8 enhances radiosensitivity of a non-small cell lung cancer cell line A549.

Zhou H, Mu X, Chen J, Liu H, Shi W, Xing E, Yang K, Wu G
International journal of radiation biology 2013 Sep;89(9):708-15

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10723-1-AP(KU70,XRCC6 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 10723-1-AP(KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded rat testis using 10723-1-AP(KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue