- HPA047549 - Atlas Antibodies XRCC6 antibody

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Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
Main image
Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting XRCC6 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting XRCC6 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:118
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: s5455
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no 2: s5456
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 3: s5457
KD Reagent Page 3: KD Reagent Page 3
Antibody Lot Number: R44711
Appendix: 565ef1309cde4.pdf

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC6 antibody. Remaining relative intensity is presented.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC6 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Western blot analysis using Anti-XRCC6 antibody HPA047549 (A) shows similar pattern to independent antibody HPA062226 (B).
Antibody #2 product nr: HPA062226
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG

Enhanced validation

Submitted by: 55af80e3e0991
Enhanced method: Genetic validation
Main image
Experimental details: Confocal images of immunofluorescently stained human U-2 OS cells.The protein XRCC6 is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where XRCC6 has been downregulated with specific siRNA.
Sample type: U-2 OS cells
Primary Ab dilution: 1:53
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:800
Knockdown/Genetic Approaches Application: Immunocytochemistry
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: s5455
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no 2: s5456
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Page 3: KD Reagent Page 3
KD Reagent Prod no 3: s5457
Antibody Lot Number: R44711
Appendix: 55b88f4520d28.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human testis and liver tissues using Anti-XRCC6 antibody. Corresponding XRCC6 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex, kidney, skin and testis using Anti-XRCC6 antibody HPA047549 (A) shows similar protein distribution across tissues to independent antibody HPA062226 (B).
Antibody #2 product nr: HPA062226
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells and myoepithelial cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex using Anti-XRCC6 antibody HPA047549.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin using Anti-XRCC6 antibody HPA047549.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-XRCC6 antibody HPA047549.
Sample type: HUMAN

HPA047549