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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324799 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal XRCC6 Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal XRCC6 Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- XRCC6
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of XRCC6 Antibody (C-term) (Cat. #TA324799) in A2058 cell line lysates (35ug/lane).XRCC6 (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of XRCC6 (arrow) using rabbit polyclonal XRCC6 Antibody (C-term) (Cat. #TA324799). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XRCC6 gene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of XRCC6 Antibody (C-term)(Cat#TA324799) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with XRCC6 Antibody (C-term)(Cat#TA324799). Hela cells were incubated with XRCC6 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- XRCC6 Antibody (C-term) (Cat. #TA324799) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- XRCC6 Antibody (C-term) (Cat. #TA324799) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
