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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00002547-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002547-M01, RRID:AB_425443
- Product name
- XRCC6 monoclonal antibody (M01), clone 4C2-1A6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant XRCC6.
- Antigen sequence
MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGR
DSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSV
YISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVL
QELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSD
YSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNP
HGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGG
FDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRK
VRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKA
LKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSD
TKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGF
KPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSAL
LIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEEL
DDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMAT
PEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNL
EALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEF
KELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYS
EEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLK
KQELLEALTKHFQD- Isotype
- IgG
- Antibody clone number
- 4C2-1A6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references DNA-PKcs expression predicts response to radiotherapy in prostate cancer.
Myonuclear breakdown in sporadic inclusion body myositis is accompanied by DNA double strand breaks.
Bouchaert P, Guerif S, Debiais C, Irani J, Fromont G
International journal of radiation oncology, biology, physics 2012 Dec 1;84(5):1179-85
International journal of radiation oncology, biology, physics 2012 Dec 1;84(5):1179-85
Myonuclear breakdown in sporadic inclusion body myositis is accompanied by DNA double strand breaks.
Nishii M, Nakano S, Nakamura S, Wate R, Shinde A, Kaneko S, Kusaka H
Neuromuscular disorders : NMD 2011 May;21(5):345-52
Neuromuscular disorders : NMD 2011 May;21(5):345-52
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of XRCC6 expression in transfected 293T cell line by G22P1 monoclonal antibody (M01), clone 4C2-1A6.Lane 1: XRCC6 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- XRCC6 monoclonal antibody (M01), clone 4C2-1A6 Western Blot analysis of XRCC6 expression in C32 ( Cat # L002V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged XRCC6 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to XRCC6 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to XRCC6 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
