H00005170-B01P

antibody from Abnova Corporation

Targeting: PDPK1 PDK1

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005170-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005170-B01P, RRID:AB_10718777

Product name

PDPK1 purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human PDPK1 protein.

Antigen sequence

MARTTSQLYDAVPIQSSVVLCSCPSPSMVRTQTES
STPPGIPGGSRQGPAMDGTAAEPRPGAGSLQHAQP
PPQPRKKRPEDFKFGKILGEGSFSTVVLARELATS
REYATRANSFVGTAQYVSPELLTEKSACKSSDLWA
LGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFP
EKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLK
AHPFFESVTWENLHQQTPPKLTAYLPAMSEDDEDC
YGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQR
SGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQ
AGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLL
LTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFK
TFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRY
QSHPDAAVQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma.

Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PDPK1 expression in transfected 293T cell line (H00005170-T01) by PDPK1 MaxPab polyclonal antibody.Lane 1: PDPK1 transfected lysate(47.19 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in K-562.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in Raw 264.7.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)