MA5-31834

antibody from Invitrogen Antibodies

Targeting: PDPK1 PDK1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31834

Provider

Invitrogen Antibodies

Product name

PDPK1 Monoclonal Antibody (7E4G11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

7E4G11

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDPK1 in human PDPK1 recombinant protein. Sample was incubated with PDPK1 monoclonal antibody (Product # MA5-31834) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDPK1 in HEK293 (1) and PDPK1 cell lysate. Samples were incubated with PDPK1 monoclonal antibody (Product # MA5-31834) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDPK1 in MCF-7 (1), HeLa (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate. Samples were incubated with PDPK1 monoclonal antibody (Product # MA5-31834) using a dilution of 1:500-1:2000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PDPK1 in A549 cells (green). Sample was incubated with PDPK1 monoclonal antibody (Product # MA5-31834) using a dilution of 1:50-1:200 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PDPK1 in paraffin-embedded bladder cancer tissue. Sample was incubated with PDPK1 monoclonal antibody (Product # MA5-31834) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PDPK1 in A549 cells (green). Sample was incubated with PDPK1 monoclonal antibody (Product # MA5-31834) using a dilution of 1:200-1:400 followed by negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of PDPK1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with PDPK1 monoclonal antibody (Product # MA5-31834) using a dilution of 1:10,000.