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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- 700044 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PLCG1 (Tyr783) Recombinant Rabbit Monoclonal Antibody (C-p1p2-39H16L83)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse, rat, Xenopus and zebrafish based on sequence homology.
- Antibody clone number
- C-p1p2-39H16L83
- Concentration
- 0.5 mg/mL
Submitted references Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.
Glucocorticoid receptors recruit the CaMKIIα-BDNF-CREB pathways to mediate memory consolidation.
Zikherman J, Doan K, Parameswaran R, Raschke W, Weiss A
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 3;109(1):E3-12
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 3;109(1):E3-12
Glucocorticoid receptors recruit the CaMKIIα-BDNF-CREB pathways to mediate memory consolidation.
Chen DY, Bambah-Mukku D, Pollonini G, Alberini CM
Nature neuroscience 2012 Dec;15(12):1707-14
Nature neuroscience 2012 Dec;15(12):1707-14
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PLC gamma pTyr783 in untreated NIH-3T3 cell lysate or treated with 50 ng/mL PDGF for 30 min (lanes 2-4) using a Phospho-PLC gamma pTyr783 recombinant rabbit monoclonal antibody (Product # 700044).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho-PLCG1 (Tyr783) was performed using 70% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-PLCG1 (Tyr783) Antibody (C-p1p2-39H16L83), Recombinant Rabbit Monoclonal (Product # 700044) at 2 µg/mL in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2,000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel f represents cells treated with antagonist, Afatinib (1 µM for 6 hrs) followed by EGF (200 ng/mL for 10 minutes), showing reduced Phospho-PLCG2 (Tyr1217) staining. Panel e shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Phospho-PLC gamma pTyr783 in NIH-3T3 cells using a Phospho-PLC gamma pTyr783 recombinant rabbit monoclonal antibody (Product # 700044) at a dilution of 3ug. Cells were fixed and permeabilized using FIX & PERM (Product # GAS004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (black) compared to a control without primary antibody (gray). Samples were preincubated for 30 minutes in the presence of 50ug/ml of the specific phosphopeptide (red) as well as the non-phosphopeptide (blue) to show specificity for the phosphopeptide only.
