NBP2-52532

antibody from Novus Biologicals

Targeting: PLCG1 NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-52532

Provider

Novus Biologicals

Product name

Mouse Monoclonal PLC-gamma 1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified.

Reactivity

Human, Rat, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Western blot analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 1192-1291)-hIgGFc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis using PLCG1 mouse mAb against Hela (1), A431 (2), C6 (3), NIH/3T3 (4), COS7 (5), and HCT116 (6) cell lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis using PLCG1 mAb against human PLCG1 (AA: 1192-1291) recombinant protein. (Expected MW is 37.5 kDa)

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of paraffin-embedded cervical cancer tissues using PLCG1 mouse mAb with DAB staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of paraffin-embedded bladder cancer tissues using PLCG1 mouse mAb with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of Jurkat cells using PLCG1 mouse mAb (green) and negative control (red).