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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-5283 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-5283, RRID:AB_628120
- Product name
- Anti-PLCG2
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.
Syk-mediated translocation of PI3Kdelta to the leading edge controls lamellipodium formation and migration of leukocytes.
Phospholipase C-gamma1 is required for the activation of store-operated Ca2+ channels in liver cells.
Targeting of protein kinase C-epsilon during Fcgamma receptor-dependent phagocytosis requires the epsilonC1B domain and phospholipase C-gamma1.
Chen Y, Wang X, Di L, Fu G, Chen Y, Bai L, Liu J, Feng X, McDonald JM, Michalek S, He Y, Yu M, Fu YX, Wen R, Wu H, Wang D
The Journal of biological chemistry 2008 Oct 24;283(43):29593-601
The Journal of biological chemistry 2008 Oct 24;283(43):29593-601
Syk-mediated translocation of PI3Kdelta to the leading edge controls lamellipodium formation and migration of leukocytes.
Schymeinsky J, Then C, Sindrilaru A, Gerstl R, Jakus Z, Tybulewicz VL, Scharffetter-Kochanek K, Walzog B
PloS one 2007 Nov 7;2(11):e1132
PloS one 2007 Nov 7;2(11):e1132
Phospholipase C-gamma1 is required for the activation of store-operated Ca2+ channels in liver cells.
Litjens T, Nguyen T, Castro J, Aromataris EC, Jones L, Barritt GJ, Rychkov GY
The Biochemical journal 2007 Jul 15;405(2):269-76
The Biochemical journal 2007 Jul 15;405(2):269-76
Targeting of protein kinase C-epsilon during Fcgamma receptor-dependent phagocytosis requires the epsilonC1B domain and phospholipase C-gamma1.
Cheeseman KL, Ueyama T, Michaud TM, Kashiwagi K, Wang D, Flax LA, Shirai Y, Loegering DJ, Saito N, Lennartz MR
Molecular biology of the cell 2006 Feb;17(2):799-813
Molecular biology of the cell 2006 Feb;17(2):799-813
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 148
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
