Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX102017 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102017, RRID:AB_1241409
- Product name
- TRAP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references The Caveolin-3 G56S sequence variant of unknown significance: Muscle biopsy findings and functional cell biological analysis.
Tenofovir and adefovir down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity.
Brauers E, Roos A, Kollipara L, Zahedi RP, Beckmann A, Mohanadas N, Bauer H, Häusler M, Thoma S, Kress W, Senderek J, Weis J
Proteomics. Clinical applications 2017 Jan;11(1-2)
Proteomics. Clinical applications 2017 Jan;11(1-2)
Tenofovir and adefovir down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity.
Zhao X, Sun K, Lan Z, Song W, Cheng L, Chi W, Chen J, Huo Y, Xu L, Liu X, Deng H, Siegenthaler JA, Chen L
Scientific reports 2017 Apr 11;7:46344
Scientific reports 2017 Apr 11;7:46344
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX102017 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:Hep G2(GTX27900) B:MOLT4(GTX27912)7.5% SDS PAGEGTX102017 diluted at 1:1500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody (GTX102017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRAP1 antibody detects TRAP1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: TRAP1 protein stained by TRAP1 antibody (GTX102017) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRAP1 antibody detects TRAP1 protein at mitochondrion on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. TRAP1 antibody (GTX102017) dilution: 1:500.