sc-7273

antibody from Santa Cruz Biotechnology

Targeting: PPARG NR1C3, PPARG1, PPARG2, PPARgamma

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-7273

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-7273, RRID:AB_628115

Product name

Anti-PPARG

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

References

Submitted references

Cross talk between insulin and bone morphogenetic protein signaling systems in brown adipogenesis.

Zhang H, Schulz TJ, Espinoza DO, Huang TL, Emanuelli B, Kristiansen K, Tseng YH
Molecular and cellular biology 2010 Sep;30(17):4224-33

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MED14 tethers mediator to the N-terminal domain of peroxisome proliferator-activated receptor gamma and is required for full transcriptional activity and adipogenesis.

Grøntved L, Madsen MS, Boergesen M, Roeder RG, Mandrup S
Molecular and cellular biology 2010 May;30(9):2155-69

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Expression of the PAX8/PPARγ Fusion Protein Is Associated with Decreased Neovascularization In Vivo: Impact on Tumorigenesis and Disease Prognosis.

Reddi HV, Madde P, Marlow LA, Copland JA, McIver B, Grebe SK, Eberhardt NL
Genes & cancer 2010 May;1(5):480-492

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PPARdelta is a fatty acid sensor that enhances mitochondrial oxidation in insulin-secreting cells and protects against fatty acid-induced dysfunction.

Ravnskjaer K, Frigerio F, Boergesen M, Nielsen T, Maechler P, Mandrup S
Journal of lipid research 2010 Jun;51(6):1370-9

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Map4k4 negatively regulates peroxisome proliferator-activated receptor (PPAR) gamma protein translation by suppressing the mammalian target of rapamycin (mTOR) signaling pathway in cultured adipocytes.

Guntur KV, Guilherme A, Xue L, Chawla A, Czech MP
The Journal of biological chemistry 2010 Feb 26;285(9):6595-603

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Multiple roles for the non-coding RNA SRA in regulation of adipogenesis and insulin sensitivity.

Xu B, Gerin I, Miao H, Vu-Phan D, Johnson CN, Xu R, Chen XW, Cawthorn WP, MacDougald OA, Koenig RJ
PloS one 2010 Dec 2;5(12):e14199

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Epidermis-type lipoxygenase 3 regulates adipocyte differentiation and peroxisome proliferator-activated receptor gamma activity.

Hallenborg P, Jørgensen C, Petersen RK, Feddersen S, Araujo P, Markt P, Langer T, Furstenberger G, Krieg P, Koppen A, Kalkhoven E, Madsen L, Kristiansen K
Molecular and cellular biology 2010 Aug;30(16):4077-91

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Histone H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesis.

Wang L, Jin Q, Lee JE, Su IH, Ge K
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 20;107(16):7317-22

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Peroxisome proliferator-activated receptor gamma regulates expression of the anti-lipolytic G-protein-coupled receptor 81 (GPR81/Gpr81).

Jeninga EH, Bugge A, Nielsen R, Kersten S, Hamers N, Dani C, Wabitsch M, Berger R, Stunnenberg HG, Mandrup S, Kalkhoven E
The Journal of biological chemistry 2009 Sep 25;284(39):26385-93

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High efficiency lipid-based siRNA transfection of adipocytes in suspension.

Kilroy G, Burk DH, Floyd ZE
PloS one 2009 Sep 11;4(9):e6940

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IL-6 deficiency attenuates murine diet-induced non-alcoholic steatohepatitis.

Mas E, Danjoux M, Garcia V, Carpentier S, Ségui B, Levade T
PloS one 2009 Nov 20;4(11):e7929

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The peroxisome proliferator-activated receptor gamma/retinoid X receptor alpha heterodimer targets the histone modification enzyme PR-Set7/Setd8 gene and regulates adipogenesis through a positive feedback loop.

Wakabayashi K, Okamura M, Tsutsumi S, Nishikawa NS, Tanaka T, Sakakibara I, Kitakami J, Ihara S, Hashimoto Y, Hamakubo T, Kodama T, Aburatani H, Sakai J
Molecular and cellular biology 2009 Jul;29(13):3544-55

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Histone methylation regulator PTIP is required for PPARgamma and C/EBPalpha expression and adipogenesis.

Cho YW, Hong S, Jin Q, Wang L, Lee JE, Gavrilova O, Ge K
Cell metabolism 2009 Jul;10(1):27-39

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Mat1 inhibits peroxisome proliferator-activated receptor gamma-mediated adipocyte differentiation.

Helenius K, Yang Y, Alasaari J, Mäkelä TP
Molecular and cellular biology 2009 Jan;29(2):315-23

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Peroxisome proliferator-activated receptor-gamma regulates the expression of alveolar macrophage macrophage colony-stimulating factor.

Bonfield TL, Thomassen MJ, Farver CF, Abraham S, Koloze MT, Zhang X, Mosser DM, Culver DA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):235-42

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Alternative mechanisms by which mediator subunit MED1/TRAP220 regulates peroxisome proliferator-activated receptor gamma-stimulated adipogenesis and target gene expression.

Ge K, Cho YW, Guo H, Hong TB, Guermah M, Ito M, Yu H, Kalkum M, Roeder RG
Molecular and cellular biology 2008 Feb;28(3):1081-91

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Impaired adipogenesis caused by a mutated thyroid hormone alpha1 receptor.

Ying H, Araki O, Furuya F, Kato Y, Cheng SY
Molecular and cellular biology 2007 Mar;27(6):2359-71

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Peroxisome proliferator-activated receptor subtype- and cell-type-specific activation of genomic target genes upon adenoviral transgene delivery.

Nielsen R, Grøntved L, Stunnenberg HG, Mandrup S
Molecular and cellular biology 2006 Aug;26(15):5698-714

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The Wnt/beta-catenin signaling pathway targets PPARgamma activity in colon cancer cells.

Jansson EA, Are A, Greicius G, Kuo IC, Kelly D, Arulampalam V, Pettersson S
Proceedings of the National Academy of Sciences of the United States of America 2005 Feb 1;102(5):1460-5

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Noradrenaline represses PPAR (peroxisome-proliferator-activated receptor) gamma2 gene expression in brown adipocytes: intracellular signalling and effects on PPARgamma2 and PPARgamma1 protein levels.

Lindgren EM, Nielsen R, Petrovic N, Jacobsson A, Mandrup S, Cannon B, Nedergaard J
The Biochemical journal 2004 Sep 1;382(Pt 2):597-606

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Jagged-1 mediated activation of notch signaling induces complete maturation of human keratinocytes through NF-κB and PPARγ

B J Nickoloff, J-Z Qin, V Chaturvedi, M F Denning, B Bonish, L Miele
Cell Death and Differentiation 2002 Jul;9(8):842-855

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 54

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human oral mucosa shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.