683402

antibody from BioLegend

Targeting: PPARG NR1C3, PPARG1, PPARG2, PPARgamma

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

683402

Provider

BioLegend

Proper citation

BioLegend Cat#683402, RRID:AB_2572020

Product name

Purified anti-PPARγ

Antibody type

Monoclonal

Antigen

Synthetic PPARγ peptide conjugated to KLH (CETDMSLHPLLQEIYKDLY)

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

14G4B17

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from mouse 3T3-L1 cells (lane 1), differentiated 3T3-L1 cells (lane 2), human PC3 cells (lane 3), and human THP-1 cells (lane 4) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human/mouse PPARγ (clone 14G4B17) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody and chemiluminescence detection.

Conjugate

Unconjugated