Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA347270 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal PPARG Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal PPARG Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PPARG
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 1.07 ?g/?l
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against PPARG. The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Image shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (in kDa) is shown on the left.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human PPARG. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:70,250.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP was performed on macrophages derived from mouse bone marrow using the ab against PPARG and optimized PCR primer sets for qPCR. Sheared chromatin from 1 million cells and 1 ug of PPARg antibody were used per ChIP experiment. IgG was used as a negative IP control. Figure 1A: recovery, expressed as the % of input, of the PDK4 PPAR response element (RE). Figure 1B: recovery of the FABP4 Adipo PPAR RE in cells treated with RSG, a very strong activating ligand of PPARG, and in untreated cells.
- Validation comment
- Assay
