PA1-824

antibody from Invitrogen Antibodies

Targeting: PPARG NR1C3, PPARG1, PPARG2, PPARgamma

Provider product page for PA1-824

Western blot

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [23]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [6]
Other assay [4]

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Product number: PA1-824 - Provider product page
Provider: Invitrogen Antibodies
Product name: PPAR gamma-2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-824 detects peroxisome proliferator activated receptor (PPAR) gamma 2 from human and mouse tissues. This antibody does not detect PPAR alpha or PPAR delta. PA1-824 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate. PA1-824 inhibits PPAR gamma 2 DNA binding. PA1-824 immunogen is a synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2. This sequence is not conserved in PPAR gamma-1. PA1-824 can be used with blocking peptide PEP-027.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

PPARG protein structure - PA1-824 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PPAR gamma-2 (green) showing positive staining in the nucleus and cytoplasm of 3T3-L1 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a PPAR gamma-2 polyclonal antibody (Product # PA1-824) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PPAR gamma-2 (green) showing positive staining in the nucleus and cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a PPAR gamma-2 polyclonal antibody (Product # PA1-824) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PPAR gamma-2 (green) showing positive staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a PPAR gamma-2 polyclonal antibody (Product # PA1-824) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PPAR gamma (red) in 3T3-L1 differentiated day 7 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a PPAR gamma rabbit polyclonal antibody (Product # PA1-824) at a dilution of 1:200 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Dylight 680 (Product # 35568) at a dilution of 1:1000 for at least 30 minutes at room temperature in the dark (red). Lipids (green) were stained with HCS LipidTOX neutral lipid stain (Product # H34475) at a dilution of 1:200 for at least 30 minutes at room temperature in the dark. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a EVOS FL Auto Imaging System at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PPAR gamma (green) in 3T3-L1 Undifferentiated (left panel) and Differentiated day 7 cells (right panel. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a PPAR gamma rabbit polyclonal antibody (Product # PA1-824) at a dilution of 1:200 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for at least 30 minutes at room temperature in the dark. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a EVOS FL Auto Imaging System at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PPAR gamma (green) in 3T3-L1 Undifferentiated (left panel) and Differentiated day 7 cells (right panel. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a PPAR gamma rabbit polyclonal antibody (Product # PA1-824) at a dilution of 1:200 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for at least 30 minutes at room temperature in the dark. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a EVOS FL Auto Imaging System at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 6 Heterogeneity of intrathymic LLMC phenotypes. A : Left panel: Preadipocytes immunostained for PPARgamma2 in the thymic parenchyma close to the capsule and blood vessels. Small panels show representative photomicrographs of intrathymic lipid-laden preadipocytes immunolabeled for PPARgamma2 (green) or PLIN (red); merged images in the right panel. B: Left panel shows thymic brown adipocytes expressing UCP-1 near blood vessels in the parenchyma. Small panels show representative photomicrographs of intrathymic brown adipocytes immunolabeled for UCP1 (green) or PLIN (red); merged images in the right panel C: intrathymic macrophagic LLMC expressing PLIN (red) and Iba 1 (green). D: Pericytic LLMC (white arrowheads) expressing PLIN (red) and NG2 (green) adjacent to the capsule. Insets show higher magnification of perilipin and NG2 staining around the lipid droplets of the pericytic LLMC. E : myofibroblastic LLMC stained with antibody for PLIN (red) and alphaSMA (green) surrounding a blood vessel. F: Thymic epithelial cells positively stained with antibody anti-Pan cytokeratin (CK, green) did not express PLIN (red). Nuclei stained with DAPI or Hoechst dye (blue). BV: blood vessels, Ca: capsule, S: septae. Scale bars in A, left panels: 15mum; right panel: 7 mum in B, left panels: 25mum; right panel: 5mum; in C: 10mum; in D: 20mum, inset: 10mum; in E: 15mum; in F: 30mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 3 The truncated PPARgamma2 localizes in the mitochondrial matrix. (A) The truncated form of PPARgamma2 in mitochondria is protected from proteinase K digestion. Purified brown adipose tissue mitochondria (60 mug) were treated with increasing amounts of proteinase K in the absence or presence of 1% Triton X-100. (B) Immuno-transmission electron microscopic analysis of the truncated PPARgamma2 in brown adipocytes. Black dots indicated by arrow heads represent immunogold particles reacted with PPARgamma (H100) antibody. Mitochondrial localization of immunogold particles was examined in 4-5 grids per group (20-30 mitochondria/grid), and the relative number of immunogold particles localized in the mitochondria was shown in the right panel. Data are presented as the mean +- SEM. Data represent mean +-SEM. ****P