Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-19853 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PKNOX1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references MiR-17 family-mediated regulation of Pknox1 influences hepatic steatosis and insulin signaling.
Ye D, Lou G, Zhang T, Dong F, Liu Y
Journal of cellular and molecular medicine 2018 Dec;22(12):6167-6175
Journal of cellular and molecular medicine 2018 Dec;22(12):6167-6175
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKNOX1 Antibody [NBP2-19853] - Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 10% SDS PAGE gel, diluted at 1:2000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PKNOX1 Antibody [NBP2-19853] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 ug of PKNOX1 antibody 10% SDS-PAGE The immunoprecipitated PKNOX1 protein was detected by PKNOX1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
