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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX114991 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114991, RRID:AB_11171103
- Product name
- PKNOX1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 10% SDS PAGE GTX114991 diluted at 1:2000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PKNOX1 protein stained by PKNOX1 antibody (GTX114991) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PKNOX1 antibody immunoprecipitates PKNOX1 ?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 ?protein by 2 £gg of PKNOX1 ?antibody (GTX114991) 10% SDS-PAGE The immunoprecipitated PKNOX1 ?protein was detected by PKNOX1 ?antibody (GTX114991) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
